Jumat, 24 Oktober 2008

ENzim Restrisi

Tugas Bioteknologi
Nama Enzim Restriksi dan Lokasinya

Nama : Taufik Hidayat
NIM : 050250
Kelas : B

No.
Nama Enzim
Organisme Asal
Sekuen Pengenal
Cut
1
EcoRI
Escherichia coli
5'GAATTC3'CTTAAG
5'---G AATTC---3'3'---CTTAA G---5'
2
EcoRII
Escherichia coli
5'CCWGG3'GGWCC
5'--- CCWGG---3'3'---GGWCC ---5'
3
BamHI
Bacillus amyloliquefaciens
5'GGATCC3'CCTAGG
5'---G GATCC---3'3'---CCTAG G---5'
4
HindIII
Haemophilus influenzae
5'AAGCTT3'TTCGAA
5'---A AGCTT---3'3'---TTCGA A---5'
5
TaqI
Thermus aquaticus
5'TCGA3'AGCT
5'---T CGA---3'3'---AGC T---5'
6
NotI
Nocardia otitidis
5'GCGGCCGC3'CGCCGGCG
5'---GC GGCCGC---3'3'---CGCCGG CG---5'
7
HinfI
Haemophilus influenzae
5'GANTC3'CTNAG
5'---G ANTC---3'3'---CTNA G---5'
8
Sau3A
Staphylococcus aureus
5'GATC3'CTAG
5'--- GATC---3'3'---CTAG ---5'
9
PovII*
Proteus vulgaris
5'CAGCTG3'GTCGAC
5'---CAG CTG---3'3'---GTC GAC---5'
10
SmaI*
Serratia marcescens
5'CCCGGG3'GGGCCC
5'---CCC GGG---3'3'---GGG CCC---5'
11
HaeIII*
Haemophilus aegyptius
5'GGCC3'CCGG
5'---GG CC---3'3'---CC GG---5'
12
AluI*
Arthrobacter luteus
5'AGCT3'TCGA
5'---AG CT---3'3'---TC GA---5'
13
EcoRV*
Escherichia coli
5'GATATC3'CTATAG
5'---GAT ATC---3'3'---CTA TAG---5'
14
KpnI[29]
Klebsiella pneumoniae
5'GGTACC3'CCATGG
5'---GGTAC C---3'3'---C CATGG---5'
15
PstI[29]
Providencia stuartii
5'CTGCAG3'GACGTC
5'---CTGCA G---3'3'---G ACGTC---5'
16
SacI[29]
Streptomyces achromogenes
5'GAGCTC3'CTCGAG
5'---GAGCT C---3'3'---C TCGAG---5'
17
SalI[29]
Streptomyces albus
5'GTCGAC3'CAGCTG
5'---G TCGAC---3'3'---CAGCT G---5'
18
ScaI[29]
Streptomyces caespitosus
5'AGTACT3'TCATGA
5'---AGT ACT---3'3'---TCA TGA---5'
19
SphI[29]
Streptomyces phaeochromogenes
5'GCATGC3'CGTACG
5'---G CATGC---3'3'---CGTAC G---5'
20
StuI [30][31]
Streptomyces tubercidicus
5'AGGCCT3'TCCGGA
5'---AGG CCT---3'3'---TCC GGA---5'
21
XbaI[29]
Xanthomonas badrii
5'TCTAGA3'AGATCT
5'---T CTAGA---3'
3'---AGATC T---5'
22
BalI
Brevibacterium albidum
TGG↓CCA

23
BsuRI
Bacillus subtilis
GG↓CC

24
HhaI
Haemophilus haemolyticus
GCG↓C

25
BamNxI

GGWCC

26
AbeI

CCTCAGC


Nama Enzim
Recognition Sequence
Compatible ends(at this website)
ReactionConditions
AarI
...CACCTGCNNNNNNNNN......GTGGACGNNNNNNNNN...
None
37oC
AasI
...GACNNNNNNGTC......CTGNNNNNNCAG...
DrdI, DseDI
37oC
AatII
...GACGTC......CTGCAG...
ZraI
37oC
AccI
...GTMKAC......CAKMTG...
FblI, XmiI
37oC
AccII
...CGCG......GCGC...
Bsh1236I, BstFNI, BstUI, MvnI
37oC
AccIII
...TCCGGA......AGGCCT...
Aor13HI, BlfI, BseAI, Bsp13I, BspEI, Kpn2I, MroI
65oC
Acc16I
...TGCGCA......ACGCGT...
AviII, FspI, NsbI
37oC
Acc36I
...ACCTGCNNNNNNNNN......TGGACGNNNNNNNNN...
BfuAI, BspMI, BveI
37oC
Acc65I
...GCTACC......CCATGG...
Asp718I
37oC
AccB1I
...GGYRCC......CCRYGG...
BanI, BshNI, BspT107I
37oC
AccB7I
...CCANNNNNTGG......GGTNNNNNACC...
PflMI, Van91I
37oC
AccBSI
...CCGCTC......GGCGAG...
BsrBI, MbiI
37oC
AciI
...CCGC......GGCG...
SsiI
37oC
AclI
...AACGTT......TTGCAA...
Psp1406I
37oC
AclWI
...GGATCNNNNNN......CCTAGNNNNNN...
AlwI, BspPI
37oC
AcsI
...RAATTY......YTTAAR...
ApoI, XapI
50oC
AcuI
...CTGAAGNNNNNNNNNNNNNNNNN......GACTTCNNNNNNNNNNNNNNNNN...
Eco57I
37oC
AcvI
...CACGTG......GTGCAC...
BbrPI, Eco72I, PmaCI, PmlI, PspCI
37oC
AcyI
...GRCGYC......CYGCRG...
BsaHI, BstACI, Hin1I, Hsp92I
50oC
AdeI
...CACNNNGTG......GTGNNNCAC...
DraIII
37oC
AfaI
...GTAC......CATG...
RsaI
37oC
AfeI
...AGCGCT......TCGCGA...
Aor51HI, Eco47III
37oC
AflII
...CTTAAG......GAATTC...
BfrI, BspTI, Bst98I, MspCI, Vha464I
37oC
AflIII
...ACRYGT......TGYRCA...
None
37oC
AgeI
...ACCGGT......TGGCCA...
BshTI, CspAI, PinAI
37oC
AhdI
...GACNNNNNGTC......CTGNNNNNCAG...
AspEI, DriI, Eam1105I, EclHKI
37oC
AhlI
...ACTAGT......TGATCA...
BcuI, SpeI
37oC
AjnI
...NCCWGGN......NGGWCCN...
EcoRII, Psp6I, PspGI
55oC
AleI
...CACNNNNGTG......GTGNNNNCAC...
OliI
37oC
AluI
...AGCT......TCGA...
None
37oC
Alw21I
...GWGCWC......CWCGWG...
AspHI, Bbv12I, BsiHKAI
37oC
Alw26I
...GTCTCNNNNNN......CAGAGNNNNNN...
BsmAI
37oC
Alw44I
...GTGCAC......CACGTG...
ApaLI, VneI
37oC
AlwI
...GGATCNNNNNN......CCTAGNNNNNN...
AclWI, BspPI
37oC
AlwNI
...CAGNNNCTG......GTCNNNGAC...
CaiI
37oC
Ama87I
...CYCGRG......GTGCYC...
AvaI, BsiHKCI, BsoBI, Eco88I
37oC
Aor13HI
...TCCGGA......AGGCCT...
AccIII, BlfI, BseAI, Bsp13I, BspEI, Kpn2I, MroI
37oC
Aor51HI
...AGCGCT......TCGCGA...
AfeI, Eco47III
37oC
ApaI
...GGGCCC......CCCGGG...
None
37oC
ApaLI
...GTGCAC......CACGTG...
Alw44I, VneI
37oC
ApeKI
...GCWGC......CGWCG...
TseI
37oC
ApoI
...RAATTY......YTTAAR...
AcsI, XapI
50oC
AscI
...GGCGCGCC......CCGCGCGG...
SgsI
37oC
AseI
...ATTAAT......TAATTA...
PshBI, VspI
37oC
AsiSI
...GCGATCGC......CGCTAGCG...
SgfI
37oC
AspI
...GACNNNGTC......CTGNNNCAG...
PflFI, PsyI, Tth111I
37oC
Asp700I
...GAANNNNTTC......CTTNNNNAAG...
MroXI, PdmI, XmnI
37oC
Asp718I
...GGTACC......CCATGG...
Acc65I
37oC
AspA2I
...CCTAGG......GGATCC...
AvrII, BlnI, XmaJI
37oC
AspEI
...GACNNNNNGTC......CTGNNNNNCAG...
AhdI, DriI, Eam1105I, EclHKI
37oC
AspHI
...GWGCWC......CWCGWG...
Alw21I, Bbv12I, BsiHKAI
37oC
AspLEI
...GCGC......CGCG...
BstHHI, CfoI, HhaI
37oC
AspS9I
...GGNCC......CCNGG...
Cfr13I, Sau96I
37oC
AssI
...AGTACT......TCATGA...
ScaI, ZrmI
37oC
AsuII
...TTCGAA......AAGCTT...
Bpu14I, Bsp119I, BspT104I, BstBI, Csp45I, NspV, SfuI
37oC
AsuC2I
...CCSGG......GGSCC...
BcnI, NciI
37oC
AsuHPI
...GGTGANNNNNNNNN......CCACTNNNNNNNNN...
HphI
37oC
AsuNHI
...GCTAGC......CGATCG...
NheI
37oC
AvaI
...CYCGRG......GRGCYC...
Ama87I, BsiHKCI, BsoBI, Eco88I
37oC
AvaII
...GGWCC......CCWGG...
Bme18I, Eco47I, SinI, VpaK11BI
37oC
AviII
...TGCGCA......ACGCGT...
Acc16I, FspI, NsbI
37oC
AvrII
...CCTAGG......GGATCC...
AspA2I, BlnI, XmaJI
37oC
AxyI
...CCTNAGG......GGANTCC...
Bse21I, Bsu36I, Eco81I
37oC


Enzyme name
Recognition Sequence
Compatible ends(at this website)
ReactionConditions
BalI
...TGGCCA......ACCGGT...
MluNI, MlsI, MscI, Msp20I
37oC
BamHI
...GGATCC......CCTAGG...
None
37oC
BanI
...GGYRCC......CCRYGG...
AccB1I, BspT107I
50oC
BanII
...GRGCYC......CYCGRG...
Eco24I, EcoT38I, FriOI
37oC
BauI
...CACGAG......GTGCTC...
BshNI, BssSI, Bst2BI
37oC
BbeI
...GGCGCC......CCGCGG...
KasI, NarI, SfoI
37oC
BbrPI
...CACGTG......GTGCAC...
AcvI, Eco72I, PmaCI, PmlI, PspCI
37oC
BbsI
...GAAGACNNNNNNN......CTTCTGNNNNNNN...
BpiI, BpuAI, BstV2I
37oC
BbuI
...GCATGC......CGTACG...
PaeI, SphI
37oC
BbvI
...GCAGCNNNNNNNNNNNNN......CGTCGNNNNNNNNNNNNN...
BseXI, BstV1I
37oC
Bbv12I
...GWGCWC......CWCGWG...
Alw21I, AspHI, BsiHKAI
37oC
BbvCI
...CCTCAGC......GGAGTCG...
None
37oC
BccI
...CCATCNNNNNN......GGTAGNNNNNN...
None
37oC
BceAI
...ACGGCNNNNNNNNNNNNNNN......TGCCGNNNNNNNNNNNNNNN...
None
37oC
BciVI
...GTATCCNNNNNNN......CATAGGNNNNNNN...
BfuI
37oC
BclI
...TGATCA......ACTAGT...
FbaI, Ksp22I
50oC
BcnI
...CCSGG......GGSCC...
AsuC2I, NciI
37oC
BcuI
...ACTAGT......TGATCA...
AhlI, SpeI
37oC
BfaI
...CTAG......GATC...
FspBI, MaeI, XspI
37oC
BfmI
...CTRYAG......GAYRTC...
BpcI, BstSFI, SfcI
37oC
BfrI
...CTTAAG......GAATTC...
AflII, BspTI, Bst98I, MspCI, Vha464I
37oC
BfrBI
...ATGCAT......TACGTA...
None
37oC
BfuI
...GTATCCNNNNNNN......CATAGGNNNNNNN...
BciVI
37oC
BfuAI
...ACCTGCNNNNNNNNN......TGGACGNNNNNNNNN...
Acc36I, BspMI, BveI
50oC
BfuCI
...NGATCN......NCTAGN...
Bsp143I, BstMBI, DpnII, Kzo9I, MboI, NdeII, Sau3AI
37oC
BglI
...GCCNNNNNGGC......CGGNNNNNCCG...
None
37oC
BglII
...AGATCT......TCTAGA...
None
37oC
BlfI
...TCCGGA......AGGCCT...
AccIII, Aor13HI, BseAI, Bsp13I, BspEI, Kpn2I, MroI
not known
BlnI
...CCTAGG......GGATCC...
AspA2I, AvrII, XmaJI
37oC
BlpI
...GCTNAGC......CGANTCG...
Bpu1102I, Bsp1720I, CelII
37oC
Bme1390I
...CCNGG......GGNCC...
MspR9I, ScrFI
37oC
Bme1580I
...GKGCMC......CMCGKG...
BseSI
37oC
Bme18I
...GGWCC......CCWGG...
AvaII, Eco47I, SinI, VpaK11BI
37oC
BmgBI
...CACGTC......GTGCAG...
BtrI
37oC
BmrI
...ACTGGGNNNNNN......TGACCCNNNNNN...
BmuI
37oC
BmuI
...ACTGGGNNNNNN......TGACCCNNNNNN...
BmrI
37oC
BmtI
...GCTAGC......CGATCG...
NheI
37oC
BmyI
...GDGCHC......CHCGDG...
Bsp1286I, MhlI, SduI
37oC
BoxI
...GACNNNNGTC......CTGNNNNCAG...
BstPAI, PshAI
37oC
BpcI
...CTRYAG......GAYRTC...
BfmI, BstSFI, SfcI
not known
BpiI
...GAAGACNNNNNNN......CTTCTGNNNNNNN...
BbsI, BpuAI, BstV2I
37oC
BpmI
...CTGGAGNNNNNNNNNNNNNNNNN......GACCTCNNNNNNNNNNNNNNNNN...
GsuI
37oC
BptI
...CCWGG......GGWCC...
BseBI, Bst2UI, BstNI, BstOI, Bst2UI, MvaI
not known
Bpu10I
...CCTNAGC......GGANTCG...
None
37oC
Bpu1102I
...GCTNAGC......CGANTCG...
BlpI, Bsp1720I, CelII
37oC
Bpu14I
...TTCGAA......AAGCTT...
AsuII, Bsp119I, BspT104I, BstBI, Csp45I, Nsp V, SfuI
37oC
BpuAI
...GAAGACNNNNNNN......CTTCTGNNNNNNN...
BbsI, BpiI, BstV2I
37oC
BpuEI
...CTTGAGNNNNNNNNNNNNNNNNN......GAACTCNNNNNNNNNNNNNNNNN...
None
25oC
Sumber Pustaka :
www.wikipedia.org
www.bima.ipb.ac

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